Your wife's current situation is as follows:
Youth training of our team: Pinsorio, rugani. (2 people, if you want to fill it up, you can only drag U23 and Primavera. )
National Youth Training: De Silio, Dibala, bonucci, chiellini (chiellini is shown in FM as our team's youth training in Serie A), Beibei and Buffon. (6 people, more than two people, need to adjust two people to the unrestricted list)
Not limited to: szczesny, cuadrado, Danilo, Sandro, Ramsey, Khedira, pjanic, Zhan, Matuidi, Labio, Cristiano Ronaldo, Manjukic, Higuain and Costa. (15 people, plus two people adjusted by the National Youth League, just 17 people)
U2 1 Unlimited: Drigues, Demiral, pellegrini.
Non-EU (limited to two other league players per season): Demir, Danilo.
Non-EU members: Demiral, Danilo, Sandro, Bentancur, Costa and cuadrado.
According to the current Serie A standards, all your wife's players can participate in Serie A now. However, because the rules of the Champions League 17+4+4 are different from those of Serie A, your wife's lack of youth training in our team makes the Champions League list only 22 players, which also leads to some players not participating in the Champions League, which is also the focus of adjustment in the last 20 days before the transfer window closes.